Respiratory Syncytial Virus G Protein CX3C Motif Impairs Human Airway Epithelial and Immune Cell Responses

Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory infection in infants and young children and causes disease in the elderly and persons with compromised cardiac, pulmonary, or immune systems. Despite the highmorbidity rates of RSV infection, no highly effective treatment or vaccine is yet available. The RSV G protein is an important contributor to the disease process. A conserved CX3C chemokine-like motif in G likely contributes to the pathogenesis of disease. Through this motif, G protein binds to CX3CR1 present on various immune cells and affects immune responses to RSV, as has been shown in the mouse model of RSV infection. However, very little is known of the role of RSV CX3C-CX3CR1 interactions in human dis-ease. In this study, we use an in vitromodel of human RSV infection comprised of human peripheral blood mononuclear cells (PBMCs) separated by a permeable membrane from human airway epithelial cells (A549) infected with RSV with either an intact CX3Cmotif (CX3C) or a mutated motif (CX4C). We show that the CX4C virus induces higher levels of type I/III interferon (IFN) in A549 cells, increased IFN- and tumor necrosis factor alpha (TNF-) production by human plasmacytoid dendritic cells (pDCs) andmonocytes, and increased IFN- production in effector/memory T cell subpopulations. Treatment of CX3C virus-infected cells with the F(ab=)2 form of an anti-Gmonoclonal antibody (MAb) that blocks binding to CX3CR1 gave results similar to those with the CX4C virus. Our data suggest that the RSV G protein CX3Cmotif impairs innate and adaptive human immune responses andmay be important to vaccine and antiviral drug development. Respiratory syncytial virus (RSV) is a major cause of severebronchiolitis and pneumonia in infants and causes repeat in