Genetic Polymorphisms of UGT1A and their Association with Clinical Factors in Healthy Koreans

Glucuronidation by the uridine diphosphateglucuronosy-ltransferase 1A enzymes (UGT1As) is a major pathway for elimination of particular drugs and endogenous substances, such as bilirubin. We examined the relation of eight single nucleotide polymorphisms (SNPs) and haplotypes of the UGT1A gene with their clinical factors. For association analysis, we genotyped the variants by direct sequencing analysis and polymerase chain reaction (PCR) in 218 healthy Koreans. The frequency of UGT1A1 polymorphisms,-3279T>G,-3156G>A,-53 (TA)6>7, 211G>A, and 686C> A, was 0.26, 0.12, 0.08, 0.15, and 0.01, respectively. The frequency of-118 (T)9>10 of UGT1A9 was 0.62, which was significantly higher than that in Caucasians (0.39). Neither the-2152C>T nor the-275T>A polymorphism was observed in Koreans or other Asians in comparison wit