Neutrophil and monocyte adherence to and migration across monolayers of cytokine-activated endothelial cells: the contribution of CD18, ELAM-1

Pretreatment of endothelial cells with cytokines enhances the adherence of leukocytes, a process that is mediated by surface proteins expressed on both cell types. A three-dimensional model system for the simultaneous determina-tion of leukocyte adherence and migration was used to study the contribution of CD l l /CD18, endothelial leukocyte-adhesion molecule-1 (ELAM-1) and VLA-4 in neutrophil and monocyte adherence to and migration through cytokine-activated endothelial cells. Pretreatment of endothelial cells for 4 hours with recombinant interleukin-1s (rlL-1s) was found to enhance neutrophil adherence and migration to a much greater extent than monocyte adherence and migra-tion. Neutrophil adherence was almost completely pre-vented by the combined use of monoclonal antibodies (Mo-Abs) against ELAM-l and CD18. Although ELAM-l has bee