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Abstract. All members of Mycobacterium avium group are important pathogens for animals and humans that give granulomatous diseases. In this study we analyzed eight Mycobacterium avium subsp. avium and seven Mycobacterium avium subsp. paratuberculosis field isolates by MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem repeat) typing. The variability for MIRU-VNTR profiles was determined by MIRU-X3 and VNTR-10 for poultry isolates and MIRU-22 and MIRU-X3 loci for ruminant isolates. For 11 isolates we obtained five MIRU-VNTR different profiles, four known profiles ( INMV 106, INMV 99, INMV 100, INMV 1) and a new one (52131118) for which have not found a corresponding INMV number. The MIRU-VNTR is a biomolecular typing method easy to use for characterization and determination of Mycobacterium subspecies diversity by performing a classic PCR methodology