Evidence of a Bradykinin Bi Receptor in Human Ileum: Pharmacological Comparison to the Rabbit Aorta Bi Receptor

Accepted for publication February i4, i996 Bradykinin Bi receptors have been identified in a limited num-ber of human tissues and may have implications in pathological states of chronic inflammation. In the present study, longitudi-nal strips of postmortem human ileum displayed a strong con-tractile response to the B2 receptor agonist, bradykinin (EC5O = 7.0 nM). Noninduced ileum strips contracted only to high con-centrations (1 and 1 0 p.M) of the Bi receptor agonists, des-Arg9-BK and Lys#{176}des-Arg9-BK. After incubation overnight at 37#{176}Cthe potency of des-Arg9-BK and Lys#{176}des-Arg9-BK dra-matically increased (EC50 = 183 and 13.2 nM, respectively). The increase in Bi agonist potency was inhibited by the protein synthesis inhibitor, puromycin. Similarly, rabbit aorta strips dis-played a protein synthesis-dependent induction of the Bi ag-onist response. Incubated human ileum and rabbit aorta exhib