Genome Sequence of the Broad-Host-Range Pseudomonas Phage -S1

The broad-host-range lytic Pseudomonas phage-S1 possess a 40,192 bp double-stranded DNA (dsDNA) genome of 47 open reading frames (ORFs) and belongs to the family Podoviridae, subfamily Autographivirinae, genus T7likevirus. The lytic phage -S1 was isolated from the Greater VancouverSewage Processing plant using Pseudomonas fluorescens PW. This virus lysed most of the fluorescent Pseudomonas species tested and one strain of Pseudomonas stutzeri. Morphologically it is a member of the Podoviridae, with an icosahedral head 60 nm in diameter and short (30-nm) tails (4). The host, P. fluorescens Migula ATCC 27663, and virus (27663-B1) were purchased from the American Type Culture Collection.We have shown that-S1 is effective in killing planktonic cultures, adherent cells, and young and mature biofilms (11–13). Phage-S1 was amplified and the DNA extracted as described previously (10). The genomewas sequenced using Roche/454 rec-ommended procedures at the Plateforme d’analyses génomiques of the Institut de Biologie Intégrative et des Systèmes (Lava