Running title: Bile acids profile in liver patients

D ow nloaded from 2 Hepatobiliary diseases result in the accumulation of bile acids (BAs) in the liver, systemic blood, and other tissues leading to an unfavorable prognosis. The BA profile was characterized by the calculation of indices that describe the composition, sulfation, and amidation of total and individual BAs. Comparison of the urinary BA profiles between healthy subjects and patients with hepatobiliary diseases demonstrated significantly higher absolute concentrations of individual and total BAs in patients. The % sulfation of some individual BAs were different between the two groups. The % amidation of overall and most individual BAs was higher in patients than controls. The % of primary BAs (CDCA and CA) was higher in patients, whereas, the % of secondary BAs (DCA and LCA) was lowe