Indirect Leukocyte Migration Inhibition Reactions to a 3-M KCI Extract of Lung Adenocarcinoma by Lung Cancer Patients 1,2

ABSTRACT-Mononuclear (MN) cells from the peripheral blood of lung cancer patients were tested for their ability to respond to a 3-M KCI extract of adenocarcinoma of lung with the use of an indirect leukocyte migration inhibition (LMI) assay. Antigen-stimulated MN cell cultures were evaluated for leukocyte inhib-itory factor production by their ability to inhibit the migration of indicator polymorphonuclear cells from agarose droplets. When supernatants were prepared in conventional round-bottomed tubes (5X106 cells/tube), 25 of 44 (57%) lung cancer patients had positive indirect LMI responses to the 7661 antigen as compared to only 2 of the 30 (7%) normal donors. When supernatants were prepared in conical microtubes, with 10 times fewer MN cells, similar results were obtained. Patients with all histologic types of lung cancer had a similar incidence of reactivity, and reactivity of untreated patients did not appear t