Regulation of schistosome egg granuloma formation: host-soluble L-selectin enters tissue-trapped eggs and binds to 528

Immunogenic carbohydrate epitopes are prominent in soluble egg antigens (SEA) of Schistosoma mansoni and in vivo are released through ultramicroscopic pores in the eggshell. Previously, one immunogenic carbo-hydrate was identified as lacto-N-fucopentaose III (LNFPIII), which contains the biologically important trisaccharide Lewisx (Lex), a weak ligand for E-, L-, and P-selectins. Selectins are involved in various inflam-matory reactions, including recruitment of granulocytes. Further, L-selectin molecules are shed from leukocyte cell surfaces upon activation. These independent observations suggest that selectins may play various roles in granuloma formation and/or regulation. We tested in situ for alterations in expression of host E-, L-, or P-selectin in murine liver tissue at various times postinfection with schistosome cercariae. We found that L-selectin was expressed on cells surrounding egg granulomas, but surprisingly we also found mouse L-selectin on the surface membranes of larval miracidia within the S. mansoni ova. In contrast, neither E- nor P-selectin was found within ova. Antibodies to human or rat L-selectin or 15 other distinct mouse leukocyte surface molecules did not bind to the miracidial surface. The anti-mouse L-selectin staining of miracidia could be inhibited by wash buffer containing sulfated carbohydrates such as sulfated Lex, heparan sulfate, fucoidan, and carrageenan. The elution studies imply that the miracidial surface and, therefore, schistosomes express sulfated glycans. The binding of soluble L-selectin to miracidia was not restricted by the genetic backgroun