SNP Single nucleotide polymorphism

Lesions IN Genomes) exploits the fact that CEL I endonuclease cleaves heteroduplexes at positions of single nucleotide or small indel mismatches. To detect single nucleotide polymorphisms (SNPs) across a population, DNA pools are created and a target locus under query is PCR-amplified and subjected to heteroduplex formation, followed by CEL I cleavage. Currently, the common method used to detect cleaved products is by polyacryl-amide gel electrophoresis using a high-throughput genotyping platform. Exact SNPs are then deter-mined by sequencing. We sought to simplify the detection of CEL I-cleaved products on conven-tional agarose gels to make the technique more accessible to collaborating partners in developin