anti-CD11b, anti-CD4, anti-CD8a, and anti-CD45R/

B220. Binding of primary antibodies was detected as described (16). Percentages of inÞltrating cells were calculated from a minimum of 1000 inÞltrating cells counted on representative heart sections. 18. Supplemental web material for Fig. 2 (analysis of splenic cell populations) is available at www. sciencemag.org/feature/data/984504.shl. 19. Splenocytes were harvested 21 days after the initial immunization with FCA, ChTR1, or M7Aa, and sub-populations were analyzed by ßuorescence-activated cell sorting. SpeciÞc antibodies to rat IgG (all pur-chased from Pharmingen) were as follows: anti-CD11b, anti-CD4, anti-CD8a, and anti-CD45R/B220. 20. Spleens from M7Aa-, ChTR1-, or kka-immunized BALB/c mice (10) were removed 21 days after th