Comparison between different methods of estimation of vitamin D

Copyright © 2013 Mrinal Pal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have in-creased the need for determining vitamin D status in a convenient and cost-effective way. In order to de-termine the better method for detecting vitamin D status, we have estimated blood vitamin D in three methods such as high-pressure liquid chromatogra-phy, chemiluminescent immunoassays and enzyme immunoassays. Method: Two hundred and sixteen subjects irrespective of age and sex were studied for blood vitamin D in all the three methods over a pe-riod of 2 years. Result: The statistical analysis showed that values of vitamin D obtained by chemilumines-cent immunoassays (r = 0.978, p < 0.001) were more significantly correlated with high-pressure liquid chromatography than enzyme immunoassays (r = 0.970, p < 0.001). Conclusion: We conclude that chemiluminescent immunoassays should be used in-stead of enzyme immunoassay and high-pressure liq-uid chromatography for estimation of vitamin D