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Abstract: Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists / International Dairy Federation (AOAC / IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR). Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridin