Production and characterization of a monoclonal antibody reactive with a specific neoantigenic determinant in degradation products of fibrin and of fibrinogen

BaIb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human blood clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that pro-duces monoclonal antibodies (MoAbs) of the IgGi K type that react with a neoantigenic determinant exposed in these XDPs, but not in intact fibrinogen or in fibrin mono-mers. Furthermore, the MoAb is reactive with some pure, individual degradation products of fibrinogen (fragments X. F IBRIN is the insoluble protein matrix of a blood clot. Fibnin has a temporary function; after it has fulfilled its mole in hemostasis and tissue repair, it is proteobyzed by plasmin. This process, designated as fibninolysis, yields