Nuclear transfer to eggs and oocytes

We review experiments in which somatic cell nuclei are transplanted singly to enucleated eggs (metaphase II) in amphibia and mammals and as multiple nuclei to the germinal vesicle of amphibian oocytes (prophase I). These experiments have shown the totipotency of some somatic cell nuclei, as well as switches in cell type and changes in gene expression. Abnormalities of nuclear transplant embryo development increase greatlyas nuclei are taken from progressively more differentiated donor cells. The molecular changes that accompany the reprogramming of transplanted nuclei help to indicate the mechanisms used by eggs and oocytes to reprogram gene expression. We discuss the importance of chromosomal protein exchange, of transcription factor supply, and of chromatin access in reprogramming. Cells of the female germline are unique be-cause they form eggs that are the direct ancestors of all cells of the body including the future eggs and sperm. After normal fertiliza-tion, 100 % of eggs can form complete fertile individuals. Even when not fertilized, activated eggs of most species, especially when made dip