Label-free Quantitative Protein Profiling of vastus lateralis Muscle During Human Aging*□S

Sarcopenia corresponds to the loss of muscle mass oc-curring during aging, and is associated with a loss of muscle functionality. Proteomic links the muscle func-tional changes with protein expression pattern. To better understand the mechanisms involved in muscle aging, we performed a proteomic analysis of Vastus lateralismuscle in mature and older women. For this, a shotgun proteomic method was applied to identify soluble proteins in muscle, using a combination of high performance liquid chroma-tography and mass spectrometry. A label-free protein profiling was then conducted to quantify proteins and compare profiles from mature and older women. This analysis showed that 35 of the 366 identified proteins were linked to aging in muscle. Most of the proteins were under-represented in older compared with matur