Plasmid Profiling and Curing Analysis of Fluoroquinolone Multidrug Resistant Pseudomonas aeruginosa in Benin City

Copyright © 2013 F. A. Ehiaghe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Aim: To determine the Plasmid DNA profile of the multidrug resistant strains of Pseudomonas aeruginosa in the clini-cal isolates. Materials and Methods: Of the 150 clinical samples (Ear swab, Urine, Wound swab, Sputa and Semen) received at Lahor Research Laboratory and Medical center in Benin City, between January 2010 and December 2012, 36 (24%) yielded significant growth of P. aeruginosa. Samples were cultured on MacConkey and Blood agar. Clinical isolates were identified using standard method. Antibiotics susceptibility test employing agar disc diffusion method was used. Clinical isolates were subjected to Plasmid DNA profiling and curing test was carried out at Lahor Molecular Laboratory. This was followed by a post plasmid curing susceptibility test. Agarose gel electrophoresis was carried out to separate the Plasmid DNA using standard method. Bands were visualized using UV illuminator. Results: Woun