Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo. Cancer discovery. 2013; 3(6):616–29. doi: 10.1158/2159

ABSTRACT Bioluminescent imaging (BLI) is a powerful noninvasive tool that has dramatically accelerated the in vivo interrogation of cancer systems and longitudinal analysis of mouse models of cancer over the past decade. Various luciferase enzymes have been genetically engi-neered into mouse models (GEMM) of cancer, which permit investigation of cellular and molecular events associated with oncogenic transcription, posttranslational processing, protein–protein interactions, transformation, and oncogene addiction in live cells and animals. Luciferase-coupled GEMMs ultimately serve as a noninvasive, repetitive, longitudinal, and physiologic means by which cancer systems and ther-apeutic responses can be investigated accurately within the autochthonous context of a living animal. Signifi cance: Luciferase-dependent bioluminescence imaging coupled with genetically engineered mouse models of cancer permit interrogation of tumor biology and therapeutic response within the proper physiological context of the whole animal in vivo. Cancer Discov; 3(6); 616–29. ©2013 AACR