Original Article Effect of Prenatal Hypoxia in Transgenic Mouse Models of Preeclampsia and Fetal Growth Restriction

Mice lacking endothelial nitric oxide synthase (eNOS/) or catechol-O-methyl transferase (COMT/) exhibit a preeclampsia-like phenotype and fetal growth restriction. We hypothesized that a hypoxic insult would result in a more severe phenotype. Pregnant eNOS/, COMT/ and control (C57BL/6J) mice were randomized to hypoxic (10.5 % O2) or normal conditions (20.9 % O2) from gestational day 10.5 to 18.5. Hypoxia increased the blood pressure in all genotypes and proteinuria in C57BL/6J and eNOS/ mice. Fetal survival was significantly reduced following hypoxia, particularly in eNOS/ mice. Birth weight was decreased in both C57BL/ 6J and COMT/ mice. Placentas from COMT/ mice demonstrated increased peroxynitrite. Despite similar hypoxia-induced effects on maternal blood pressure and proteinuria, eNOS/ embryos have a decreased tolerance to hypoxia. Compared to C57BL/6J, COMT/ mice exhibited less severe changes in proteinuria and fetal growth when exposed to prenatal hypoxia. This relative resistance to prenatal hypoxia was associated with a significant increase in placental levels of peroxynitrite