Myint, Melissa, "Coordinating Gene Therapies of Cystic Fibrosis Airway Disease with Anti-Inflammatory and Antimicrobial Cationic Glucocorticoids " (2015). Publicly Accessible Penn Dissertations. Paper 1101. Coordinating Gene Therapies of Cystic Fibrosis A

Certain sterol-based cationic lipids demonstrate both anti-inflammatory and antimicrobial characteristics in addition to their abilities to facilitate gene transfer. These characteristics are particularly suited for enhancing potential gene therapies of cystic fibrosis (CF) airway disease, a condition characterized by chronic infections and concomitant inflammation. Unfortunately, however, animal models of CF do not show the airway disease typically seen in humans, so in vivo efficacies of potential therapies are difficult to assess. In this work, three mouse models were established to evaluate adeno-associated virus (AAV) gene transfer in an infected airway environment. Bordetella bronchiseptica RB50 was used in a chronic, non-lethal respiratory infection model with C57BL/6 mice. Administration of an AAV vector on day 2 of this infection resulted in an approximate three-fold reduction of reporter gene expression, compared to that observed in uninfected controls. Postponement of AAV administration to day 14 resulted in an even greater (eight-fold) reduction in gene expression. Separately, Pseudomonas aeruginosa PAO1 was used to infect surfactant protein D or surfactant protein A knockout mice to establish acute infections. Reporter gene expression was approximately ~2.5-fold lower in these infected mice than in uninfected mice, when AAV was administered on day 2 of infection. Interestingly, when AAV administration was postponed to day 9 of infection in the surfactant protein