Mechanized enzymatic determination of triglycerides in

A procedure for enzymatic determination of serum tri-glycerides [CNn. Chem. 19, 476 (1973)] has been adaptedforuse incontinuous-flowanalysis(Technicon AutoAnalyzer). A very simple manifold is used; serum is incubatedat 37 #{176}Cwith the lipase and cr-chymotrypsin inpotassium phosphate buffer (0.1 mol/liter, pH 7, Con-taining 1.50 g of bovine serum albumin per liter). The lib-erated glycerol Is dialyzed againstthecompleteglycerol reagent. The change in absorbance at 340 nm resulting from oxidation of NADH isproportionalto the dialyzed gly#{243}erol.The same manifold can be used to determine preformed glycerol if the hydrolyzing enzymes are omit-ted. The lipase used need not be extensively purified be-cause the dlalyzer removes coloring material and con-taminating enzymes that would interfere with the com