Determination of Tramadol in Human Serum by Capillary Electrophoresis with the End-Column Electrochemiluminescence Detection

Copyright © 2013 Caiyun Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A capillary electrophoresis (CE) coupled with end-column electrochemiluminescence (ECL) detection method for the analysis of tramadol (TMD) has been investigated. ECL detection was working electrode biased at 1.2 V in a 20 mmol·L−1 sodium phosphate buffer (pH = 8.0) containing 5 mmol·L−1 Ru 23bpy (where bpy = 2,2’-bipyridyl). Linear correlation (r ≥ 0.997) between ECL intensity and drug concentration was obtained in the range 3 × 10−4- 6 × 10−6 mol·L−1. The limits of detection (LODs) for tramadol in water was 3.012 × 10−8 mol·L−1 (S/N = 3). The relative standard deviation values on peak size (10−5 mol·L−1 level) and migration time for the tramadol were 4.58 % and 1.39 % (n = 10), respectively. Applicability of the CE-ECL method to the analysis of human serum spiked with tramadol was examined