Downloaded from www.microbiologyresearch.org by

The human papillomavirus type 6 (HPV-6) E4 gene was expressed in Escherichia coli as a fusion protein with E. coli fl-galactosidase (E4-/3-Gal), and rabbit antibody against the E4-/3-Gal was prepared. By Western blotting with this antibody, we detected E4 gene products in six out of 18 condyloma cuminata specimens. In four specimens (C-l, C-13, C-14 and C-19), the E4 protein was found as a 10K / l lK doublet, but in other specimens (C-8 and C-23), only the 11K protein was detected. BySouthern blot analysis, it wa