Injection Solvent Effect on Peak Height in Ion Exchange HPLC

A study of the effect of injection solvent on the peak height of simple organic anions is performed on a styrene-divinylbenzene copolymeric anion exchanger. When the injection solvent is stronger than the mobile phase, the peak is broadened, while a taller peak is obtained when a weaker injection solvent is used. The effects of volume injected and difference in strength of two solvents are also investigated. In this paper, we show the results obtained in ion exchange HPLC. The effects of injection solvent strength and injection volume on peak height were investigated on a styrene-divinyl-benzene copolymeric anion exchanger. Phenylacetate anion was chosen as a primary eluite. Solvent strength was varied using dif fering ion chromatography strength, i.e., ion kind or ion con centration, or by differing acetonitrile volume percent