Draft Genome Sequence of Paenisporosarcina sp. Strain TG-20, a Psychrophilic Bacterium Isolated from the Basal Ice of Taylor Glacier

We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 pro-tein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environments. Paenisporosarcina sp. strain TG-20was isolated from samples ofdebris-rich basal ice that were recovered via a tunnel excavated into the northmargin of theTaylorGlacier, Antarctica. Recent stud-ies have shown that glacial ice and subglacial environments contain viableanddiversepsychrophilicmicroorganisms(8).Glacial icepres-ents auniquehabitat,where entrappedmicroorganismsmustpossess variousmechanisms to survive a variety of challenges, including con-stant subzero temperatures, high osmotic stress, damaging ice crystal formation, and extremely low pH (6). Since glacial microorganisms persist in a viable state under these conditions, they require cold-adapted proteins and may possess molecular adaptations that en-hance their survival in ice (e.g., ice-binding proteins) (3, 4). Individual bacterial colonies were isolated from agar-solidifie