Printed in Great Britain Target discrimination in regenerating insect sensory nerve

The paired abdominal cerci of the cricket Acheta domesticus are mechanosensory append-ages which regenerate readily when amputated during larval life. Their peripherally-located sense cells form axons which project centrally as a purely sensory nerve to the terminal abdominal ganglion. In an attempt to analyze some of the factors which guide a regenerating sensory nerve to correct central terminations, implants of homologous, supernumerary terminal ganglia were made in cricket larvae and the host cerci amputated. The possibility that implants with multiple nerve stumps might release an attracting substance was considered. Surgical pro-cedures used were (1) implant in posterior abdomen; (2) implant in posterior abdomen, ipsilateral to chronic cereal deprivation; (3) implant in mesothoracic leg socket, adjacent to heterotopically-transplanted regenerated cercus; (4) implant in posterior abdomen, ipsi-lateral host cereal motor nerve sectioned; (5) implant in posterior abdomen, ipsilateral margin of host terminal ganglion wounded. Results were determined after the adult molt, by con-ventional histology or by cobalt chloride filling of regenerated cereal nerves. In all procedures except (3) and (4), the regenerated afferent nerve bypassed the implant and terminated in the host terminal ganglion. In (3), the regenerated fibers from cereal grafts bypassed the implant; terminations were not found. In (4), some regenerated cereal axons connected with the implant and the majority terminated in the host ganglion. It is suggested that regenerating cereal afferents may depend in a facultative way on the cereal motor nerve as a pathway guide but there is as yet no clear evidence for a trophic influence from the central nervous system