OPEN ACCESS AJAC Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica (Linn.) Using HPTLC

Copyright © 2014 Chinthala Praveena, Ciddi Veeresham. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Chinthala Praveena, Ciddi Veeresham. All Copyright © 2014 are guarded by law and by SCIRP as a guardian. Toddalia asiatica Linn. is an important medicinal plant belonging to the family Rutaceae. The plant is well known for its antimalarial activity, which has been attributed to the presence of benzophenanthridine alkaloid nitidine in the roots of plants. A simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been developed and validated for the quantitative determination of nitidine in the dried roots and plant tissue culture extracts of T. asiatica. Nitidine was estimated at 332 nm by densitometry using Silica gel 60 F254 as sta-tionary phase and chloroform:methanol (7:1, v/v), and as mobile phase. Linearity was observed in the concen-tration range of 25- 200 ng/spot for nitidine. The limit of detection and limit of quantitation were found to be 0.026 and 0.086 ng/spot respectively for nitidine. Developed method was validated according to the ICH guide-lines with respect to precision, accuracy, specificity and robustness. The technique has been applied for the firs