Effects of Clioquinol on Metal-Triggered Amyloid-beta Aggregation Revisited

Amyloid-?? (A??) plaques are largely associated with the neuropathogenesis of Alzheimer's disease (AD). Metal ions such as Cu II and Zn II have been implicated as contributors to their formation and deposition. Metal chelators have been used to modulate metalinduced A?? aggregation. The bidentate ligand clioqulnol (CQ) presents an effective influence on metal-involved A?? aggregation, which has been explained through its metal chelation and is generally monitored by fluorescence and turbidity assays in vitro. The studies herein, however, suggest that the effects of CQ on metal-driven A?? aggregation may not be visualized accurately by both assays. Subsequently, the present work demonstrates that CQ is able to chelate metal ions from metal - A?? species and to assist, in part, in the disaggregation of A?? aggregates, but it could not completely hinder the progression of A?? aggregation.close444