Effects of hypothermia on excitatory amino acids and metabolism in stroke patients: a microdialysis study. Stroke

Background and Purpose—The objective of this study was to assess the effect of therapeutic moderate hypothermia on excitatory amino acids and metabolism by applying cerebral microdialysis in patients suffering from space-occupying middle cerebral artery infarction. Methods—This was an open, prospective, observational study of 12 patients undergoing moderate hypothermia (33°C) as rescue therapy for large, life-threatening middle cerebral artery infarction. Microdialysis probes were placed concomitantly with intracranial pressure (ICP) measuring devices in the frontal lobe of the infarcted and/or noninfarcted hemisphere. Using the CMA 600 Microdialysis Autoanalyzer, we analyzed glutamate, glycerol, pyruvate, and lactate. Results—According to follow-up cranial CT scans, 3 different compartments of microdialysis measurements could be defined. First, noninfarcted brain tissue had stable dialysate concentrations but a significant effect of hypothermia on glutamate (2.6 versus 3.6 mol/L), lactate (1.8 versus 3 mmol/L), and pyruvate (50 versus 95.8 mol/L). Second, measurements from peri-infarct tissue had a significant effect of hypothermia on glutamate (4.8 versus 12.6 mol/L), glycerol (58 versus 82 mol/L), lactate (0.7 versus 1.3 mmol/L), and pyruvate (13.3 versus 36.8 mol/L). Third, dialysate concentrations obtained from irreversibly damaged tissue were excessive for glutamate (453 mol/L), glycerol (1187 mol/L), lactate (12 mol/L), and pyruvate (4 mol/L). In this extreme compartment, no effect of hypothermia was observed