Evolutionary relationships of Heimioporus and Boletellus (Boletales), with an emphasis on Australian taxa including new species and new

Abstract. Boletellus and Heimioporus, two genera of Boletaceae with ornamented basidiospores, are shown to be distinct genera on the basis of phylogenetic analyses of nuclear ribosomal large-subunit and translation elongation-factor 1a DNA sequences. Comparison of spore ornamentation type – longitudinally ribbed in Boletellus v. punctate, alveolate-reticulate in Heimioporus – are further evidence for distinction. Analyses of multiple accessions from the Americas, Asia and Australia support the monophyly of Heimioporus and a ‘core Boletellus ’ clade, containing the type species, B. ananas (M.A.Curt.) Murrill, and approximately seven additional species. Tests of alternative phylogenetic topologies could no