Genome Analysis of a Mycoplasma hyorhinis Strain Derived from a

The complete genome of Mycoplasma hyorhinis strain MCLD has been sequenced and annotated. This genome differs by the inversion of a 14.4-kb and a 3.7-kb fragment and the deletion of a 9.9-kb fragment from M. hyorhinis strain HUB-1, isolated from swine respiratory tract. The genome revealed 778 coding sequences (CDSs), with a limited number of vlp genes encoding variable surface lipoproteins. Mycoplasma hyorhinis is a swine pathogen causing respira-tory diseases and arthritis. Additionally, it is a frequent cell culture contaminant and has recently been detected in human gastric carcinoma tissues (13). M. hyorhinis MCLD has been isolated from a primary human melanoma cell line (5). Al-though mycoplasmas were considered adherent extracellular microorganisms (10), it has been shown that M. hyorhinis MCLD invades host cells (7). Furthermore, host cells infected with M. hyorhinis MCLD showed elevated expression of a CD99 ligand on melanoma cells (5) and a marked increase in the cellular concentration of the protease inhibitor calpastati