A reaction of grassland to application of liquid fertilizer DAM 390

This theses summarizes results obtained from studying of one-year experimental pot of permanent grassland which was established in Vítězná - Záboří in 2015. There has been observed 4 levels of nitrogen fertilization on the meadow plot (without fertilizing, 30 kg N.ha-1, 60 kg N.ha-1 and 90 survey was influence of nitrogen on the change in species richness by using weight share representation of individual agrobotanical groups in the stand (grasses, legumes, other forbs). Results of this experiment were considerable affected by unfavourable weather events in the observed year. We should bear in mind the short time of this experiment. Positive unofficial effect of nitrogen on higher yield of above-ground biomass was recorded, especially by using 30 kg N.ha-1 and 60 kg N.ha-1. There is an exception at using 90 kg N.ha-1 with yield off balance. From the perspective of infuence of nitrogen on changes of species richness the increasing of doses of fertilizing has shown especially in increase proportion of grasses while present retreating herbs in the species composition of the reference stand. However results of experiment are not statistically different. We can not confirm studies which state decrease proportion of legumes in the grassland due to nitrogen fertilization from results of this experiment