Testing of bioeffectors on phosphorus mobility in soils

Crave for increase in agricultural production over the past sixty years reflects in widespread use of mineral fertilizers. Due to the growth of the world population an increase of overall demand for feed and food is expected. Limited availability of cultivatable soil and increasing dependance on mineral fertilizers can be also anticipated. It is therefore important to find alternative strategies for plant nutrition. In 2012, the project of cooperation between several universities in Europe was revealed. This project examines the use of bioeffectors in crop production. Use of these substances should help to reduce the input of mineral fertilizers used in agriculture and improve land usage. Thesis will perform testing in real conditions of different geographic locations. The main objective is to develop new approaches to the use of so-called bioeffectors based on the mechanism of action of living microorganisms and active natural substances. Bioeffectors may be, depending on soil and climatic conditions, a key factor for overcoming limitations in the availability of nutrients. They can contain microorganisms (bacteria, fungi) and active natural compounds (digest from soil or compost, microbial residues, plant extracts, metabolites from biological processes, dried herb material or seaweed). These substances are developed for a wide variety of crops (e.g. corn, wheat and tomatoes). Their effectiveness consists in nutrients mobilization from less accessible forms in the soil. The thesis is mainly focused on the impact of bioeffectors on various forms of phosphorus in the soil. Phosphorus in the soil belongs to essential elements necessary for the growth and development of plants. Despite its necessity in plant metabolism is its content in the soil relatively low. A higher phosphorus content is in most samples of soil mainly in the surface area because an increased biological activity occurs there causing the accumulation of organic material. Some influence can be also observed in connection with application of organic and mineral fertilizers. Amount of phosphorus in soil may vary depending on parental rock type, texture and land management (the ratio of P supplied and type and method of land cultivation). Above mentioned influences even have an effect on relative amount of organic and inorganic forms of phosphorus. Three basic groups of phosphorus contained in the soil can be described: inorganic phosphorus compounds, organically bound phosphorus and exchangeable absorbed phosphorus. The amount of phosphorus bound in the fractions depends mainly on the time of fertilizer application, including the impact of earlier interventions. For these reasons, it is necessary to execute detailed analysis of the various forms of phosphorus in the soil after application of mineral fertilizers together with bioeffectors. In this dissertation variety of pot and field experiments will be set up, where samples from each variation of soil and plant will be separated and researched. Samples will be analyzed and amounts of phosphorus will be measured