This bachelor thesis is concerned with the comparison between the utilization of biodiesel and diesel as fuel in truck transportation. The thesis contains a theoretical part, which describes primarily the current state of truck transportation in the Czech Republic. It further inquires into current and historic politics of biofuel support, including its impact on state budget. Also described are the most used biofuels, specifically biodiesel, whose comparison with diesel is the main subject of the thesis. The Czech market with biofuels is also discussed. The practical part includes a description of a specific transport company, particularly its vehicle fleet. The results of measurements of biodiesel consumption are further summarized, alongs...