This Bachelor's thesis aims to create an aplication for detection and recognition of license plates suitable for real-time processing. The work contains analysis of available methods. Part of the work is focused on present form of licence plates in Czech Republic. As a result of work, new data set was created and computer application was implemented. The application uses existing libraries designed for computer vision and machine learning with main purpose to detect and recognize licence plates from video. Detection is realized with help of cascade classifier, and recognition by Perceptron neural network. Final chapter subsequently contains evaluation of success rate of implemented solution