Somatic cell hybrids derived from fusion of GM637, a human cell line transformed by simian virus 40, and mouse B82 cells were examined for simian virus 40 T antigen, V antigen, and viral DNA. All hybrid cell lines that contained viral DNA were T-antigen positive. Cells that did not have viral DNA were T-antigen negative. We determined that there is a single viral insertion in these hybrid cells. Correlation of T-antigen expression and viral DNA with the partial complements of the human genome retained in the hybrids shwed that the inserted viral genome is in human chromosome 8. The integrated viral DNA is stable; free viral DNA found in GM637 does not insert at other potential sites in the human genome
Simian virus 40 (SV40) infections of semipermissive human cells and permissive monkey cells were com...
Journal ArticleWe examined the fate of DNA microinjected into nuclei of cultured mammalian cells. Th...
Just as some of the pioneers of molecular biology turned to bacteriophages because of their relative...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
Rodent cells in culture which do not support SV40 replication can be stably transformed by the virus...
When SV40 infects mouse cells, it does not replicate but instead causes neoplastic transformation of...
To examine the origin of the diverse viral integration patterns in rodent cells transformed by simia...
Human fibroblasts transformed with an adenovirus-5/simian virus 40 recombinant construct (Ad5/SV40) ...
It seems evident that in SV40-transformed mouse cells the viral DNA has combined with the host chrom...
A family of human transforming genes, previously shown to share homology with the ras family of vira...
A family of related cell lines was generated by subcloning of a hamster cell line (ev-1114-CHL-2) th...
Different methods of molecular hybridization were used to study DNA sequences of the highly oncogeni...
TO test whether the covalent linkage of simian virus 40 (SV40) A gene DNA sequences might lead to in...
Simian virus 40 (SV40) infections of semipermissive human cells and permissive monkey cells were com...
Journal ArticleWe examined the fate of DNA microinjected into nuclei of cultured mammalian cells. Th...
Just as some of the pioneers of molecular biology turned to bacteriophages because of their relative...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
The arrangement and localization of viral genomes integrated in RSb cells were analyzed by molecular...
Rodent cells in culture which do not support SV40 replication can be stably transformed by the virus...
When SV40 infects mouse cells, it does not replicate but instead causes neoplastic transformation of...
To examine the origin of the diverse viral integration patterns in rodent cells transformed by simia...
Human fibroblasts transformed with an adenovirus-5/simian virus 40 recombinant construct (Ad5/SV40) ...
It seems evident that in SV40-transformed mouse cells the viral DNA has combined with the host chrom...
A family of human transforming genes, previously shown to share homology with the ras family of vira...
A family of related cell lines was generated by subcloning of a hamster cell line (ev-1114-CHL-2) th...
Different methods of molecular hybridization were used to study DNA sequences of the highly oncogeni...
TO test whether the covalent linkage of simian virus 40 (SV40) A gene DNA sequences might lead to in...
Simian virus 40 (SV40) infections of semipermissive human cells and permissive monkey cells were com...
Journal ArticleWe examined the fate of DNA microinjected into nuclei of cultured mammalian cells. Th...
Just as some of the pioneers of molecular biology turned to bacteriophages because of their relative...