The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting
We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities...
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presen...
Quantitative real-time-PCR (q-PCR) is largely used to evaluate different pathogens in both environme...
The aims of this study were to identify the best threshold value for the real-time PCR method in det...
A comparative assessment of conventional culture and nucleic acid techniques in the detection of Leg...
A real-time LightCycler assay for Legionella pneumophila was evaluated with 120 water samples potent...
The genus Legionella comprises more than 40 species and 64 serogroups with approximately half of tho...
The aim of the present study was: – to compare methods for concentration and isolation of Legionella...
We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testin...
An epidemiologic study on the isolation of Legionella spp from the sanitary water of a public Hospit...
Waterborne diseases are a serious threat because of their ability to infect a high number of individ...
A new real-time PCR assay was developed and validated in combination with an immunomagnetic separati...
Background: In 2014, about 7,000 cases of Legionnaires' disease were reported in the European Union ...
Legionella pneumophila is an important etiological agent in both hospital and community acquired pne...
To perform an international trial to derive alert and action levels for the use of quantitative PCR ...
We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities...
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presen...
Quantitative real-time-PCR (q-PCR) is largely used to evaluate different pathogens in both environme...
The aims of this study were to identify the best threshold value for the real-time PCR method in det...
A comparative assessment of conventional culture and nucleic acid techniques in the detection of Leg...
A real-time LightCycler assay for Legionella pneumophila was evaluated with 120 water samples potent...
The genus Legionella comprises more than 40 species and 64 serogroups with approximately half of tho...
The aim of the present study was: – to compare methods for concentration and isolation of Legionella...
We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testin...
An epidemiologic study on the isolation of Legionella spp from the sanitary water of a public Hospit...
Waterborne diseases are a serious threat because of their ability to infect a high number of individ...
A new real-time PCR assay was developed and validated in combination with an immunomagnetic separati...
Background: In 2014, about 7,000 cases of Legionnaires' disease were reported in the European Union ...
Legionella pneumophila is an important etiological agent in both hospital and community acquired pne...
To perform an international trial to derive alert and action levels for the use of quantitative PCR ...
We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities...
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presen...
Quantitative real-time-PCR (q-PCR) is largely used to evaluate different pathogens in both environme...