Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Haybal