Influence of adipocyte secretions on proliferation process and tumor angiogenesis

The Second International Congress of Translational Research in Human Nutrition is organised by the Research Centre in Human Nutrition (CRNH) of Auvergne, of which INRA is a member, in collaboration with NuGO, European Association of universities and research institutes in the field of nutrigenomicsObesity, which incidence dramatically increases, is an established risk factor for breastcancer in postmenopausal women with a higher risk of poor final outcome independently of tumorstage. Adipokines, secreted by adipose tissue which surround normal or tumor mammary epithelialcells, could affect mammary tumorogenesis. Adipokines, whose plasma concentrations aremodulated in case of obesity, may have an impact on proliferation processes and tumorangiogenesis. In this context, the objective of this research project was to characterize the impact ofadipocyte secretions from obese or lean women on tumor proliferation and on the key steps ofangiogenesis, in order to better understand i) the influence of obesity on these two processes and ii)the dialogue between tumor cells and adipocytes.Mature adipocytes (MA) obtained from lean (MA20) or obese women (MA30) were cultured and theeffects of MA supernatants (Sn, Sn20 and Sn30 respectively) were evaluated in vitro on tumorproliferation (fluorescent resazurin test) and on different steps of angiogenesis (proliferation,migration and endothelial tube formation by endothelial cells HUVEC). The influence of Sn obtainedfrom co-cultures between MA20 or MA 30 and human breast cancer cells MCF-7 (respectively Sn7/20and Sn7/30) was also investigated on angiogenic process.Our preliminary results showed that the proliferation of MCF-7 was more increased in the presenceof Sn30 (50% vs. Control (T), p <0.001) than Sn20. Similarly, angiogenesis was stimulated in case ofobesity since Sn30 increased proliferation (53% vs T, p <0.001), migration (63% vs T, p