Flavin mononucleotide-exfoliated graphene flakes as electrodes for the electrochemical determination of uric acid in the presence of ascorbic acid

Gold interdigitated microelectrodes (Au-IDA) modified with either graphene flakes exfoliated using flavin mononucleotide (FMN) (Gr-FMN) or graphene flakes and platinum nanoparticles (Pt-Gr-FMN) have been studied in the oxidation of uric acid (UA). An electrochemical method for the detection and quantification of UA in phosphate buffer solution at physiological pH (PBS, 0.25 M, pH 7) in the absence and presence of ascorbic acid (AA) has been studied by cyclic voltammetry. The quantification of UA was investigated by cyclic voltammetry, presenting an oxidation peak at 0.99 V with both modified electrodes. Linearity range of 60–578 μM and 60–345 μM has been found for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA electrodes, respectively. Limits of detection of 18 μM were obtained for both electrodes, and the repeatability was studied at 177 μM providing 4% and 8% for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA, respectively. AA interference has been studied by cyclic voltammetry, showing two clearly separated oxidation peaks, at 0.99 V for UA oxidation and at 0.74 V for Gr-FMN/Au-IDA and 0.70 V for Pt-Gr-FMN/Au-IDA for AA oxidation. Linearity range has been studied in presence of 250 μM AA obtaining a working range of 60–578 μM for Gr-FMN/Au-IDA electrode and of 60–288 μM with Pt-Gr-FMN/Au-IDA electrode. Limits of detection remain at 18 μM for both electrodes and the repeatability was studied at 177 μM providing 8% and 14% for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA electrodes respectively.This work is supported by the Generalitat Valenciana (Prometeo2013/038) and by the Spanish Ministerio de Economía y Competitividad (MAT2013-42007-P). We also acknowledge partial funding of this work by Ministerio de Economía y Competitividad and the European Regional Development Fund (ERDF) through projectMAT2015-69844-R aswell as by Plan de Ciencia, Tecnología e Innovación 2013–2017 del Principado de Asturias and ERDF (project GRUPIN14-056). A. Abellán-Llobregat also thanks the Generalitat Valenciana for her fellowship and M. Ayán-Varela acknowledges his predoctoral contract (FPI) from Ministerio de Economía y Competitividad