Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
- Weierstall, Uwe
- James, Daniel
- Fromme, Raimund
- Grotjohann, Ingo
- Kupitz, Christopher
- Zatsepin, Nadia A.
- Liu, Haiguang
- Basu, Shibom
- Wacker, Daniel
- Won Han, Gye
- Katritch, Vsevolod
- Boutet, Sébastien
- Wang, Chong
- Messerschmidt, Marc
- Williams, Garth J.
- Koglin, Jason E.
- Marvin Seibert, M.
- Klinker, Markus
- Gati, Cornelius
- Shoeman, Robert L.
- Barty, Anton
- Chapman, Henry N.
- Kirian, Richard
- White, Thomas
- Beyerlein, Kenneth
- Stevens, Raymond C.
- Li, Dianfan
- Shah, Syed T. A.
- Howe, Nicole
- Caffrey, Martin
- Cherezov, Vadim
- Wang, Dingjie
- Liu, Wei
- Spence, John C. H.
- Bruce Doak, R.
- Nelson, Garrett
- Fromme, Petra
Publication date
January 2014
DOI Publisher
Macmillan Publishers Limited ISSN
2041-1723 Citation count (estimate)
217Abstract
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor
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