Add to ORKGTato práce se zabývá postsyntetickým značením modifikovaných oligonukleotidů fluoroforem pomocí konjugační reakce. Dále se věnuje výběru vhodné purifikační metody pro odstranění nenavázaného fluoroforu s následným spektrofotometrickým ověřením. Takto připravené oligonukleotidy byly poté použity jako hydrolyzační sondy v real-time PCR analýze.This work deals with postsynthetic labeling of modified oligonucleotides using fluorofore conjugation reaction. It also discusses the selection of suitable purification method to remove unbound fluorofore followed by spectrophotometric verification. The prepared oligonucleotides were then used as hydrolysis probes in real-time PCR analysis.Fakulta chemicko-technologick
Ligands can be introduced at the 5′ terminus of an oligonucleotide by adding a linker to the ligand ...
A method for the enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides was develope...
Tato práce se věnuje fluorescenci, biomolekulám a jejich značení. Fluorescence je popsána v kontextu...
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and...
There is a large requirement in modern molecular biology for the production of specifically conjugat...
In the last 30 years oligonucleotides i.e., relatively short polymers (usually 12-24 units long) bas...
Tato práce se zabývá použitím různě modifikovaných oligonukleotidů jako specifických blokátorů PCR. ...
The report examines how the market, as well as purification conditions, forpeptide-oligonucleotide c...
Analysis of the use of real-time PCR with fluorescent registration of results for gene diag-nosis of...
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chem...
The first part of the work describes a procedure of oligonucleotide purification using a reversed-ph...
The sequence-specific fluorescence labeling of nucleic acids is a prerequisite for various methods i...
The aim of the thesis was to synthesize new nucleosides, nucleotides and the corresponding DNA probe...
Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutic...
Modifed oligonucleotides are routinely employed as analytical probes for use in diagnostics, e.g. in...
Ligands can be introduced at the 5′ terminus of an oligonucleotide by adding a linker to the ligand ...
A method for the enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides was develope...
Tato práce se věnuje fluorescenci, biomolekulám a jejich značení. Fluorescence je popsána v kontextu...
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and...
There is a large requirement in modern molecular biology for the production of specifically conjugat...
In the last 30 years oligonucleotides i.e., relatively short polymers (usually 12-24 units long) bas...
Tato práce se zabývá použitím různě modifikovaných oligonukleotidů jako specifických blokátorů PCR. ...
The report examines how the market, as well as purification conditions, forpeptide-oligonucleotide c...
Analysis of the use of real-time PCR with fluorescent registration of results for gene diag-nosis of...
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chem...
The first part of the work describes a procedure of oligonucleotide purification using a reversed-ph...
The sequence-specific fluorescence labeling of nucleic acids is a prerequisite for various methods i...
The aim of the thesis was to synthesize new nucleosides, nucleotides and the corresponding DNA probe...
Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutic...
Modifed oligonucleotides are routinely employed as analytical probes for use in diagnostics, e.g. in...
Ligands can be introduced at the 5′ terminus of an oligonucleotide by adding a linker to the ligand ...
A method for the enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides was develope...
Tato práce se věnuje fluorescenci, biomolekulám a jejich značení. Fluorescence je popsána v kontextu...