Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
- Shin, Yoojin
- Han, Sewoon
- Jeon, Jessie S.
- Yamamoto, Kyoko
- Zervantonakis, Ioannis K
- Sudo, Ryo
- Kamm, Roger Dale
- Chung, Seok
- Zervantonakis, Ioannis K.
DOIAbstract
This protocol describes a simple but robust microfluidic assay combining three-dimensional (3D) and two-dimensional (2D) cell culture. The microfluidic platform comprises hydrogel-incorporating chambers between surface-accessible microchannels. By using this platform, well-defined biochemical and biophysical stimuli can be applied to multiple cell types interacting over distances of <1 mm, thereby replicating many aspects of the in vivo microenvironment. Capabilities exist for time-dependent manipulation of flow and concentration gradients as well as high-resolution real-time imaging for observing spatial-temporal single-cell behavior, cell-cell communication, cell-matrix interactions and cell population dynamics. These heterotypic cell typ...
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