Structural biology underpins rational drug design and fundamental understanding of protein function. X-ray diffraction (XRD) has been the golden standard for solving for high-resolution protein structure. Second harmonic generation (SHG) microscopy has been developed by the Simpson lab as a sensitive, crystal-specific detection method for the identification of protein crystal and help optimize the crystallization condition. Protein nanocrystals has been widely used for structure determination of membrane proteins in serial femtosecond nanocrystallography. In this thesis work, novel nonlinear optical methods were developed to address the challenges associated with the detection and characterization of protein nanocrystals. SHG-correlation sp...
Identifying and then optimizing initial crystallization conditions is a prerequisite for m...
International audienceWe have investigated nanocrystals of Fe(IO3)3 by polarization-sensitive second...
There is an increasing demand for rapid, effective methods to identify and detect protein micro- and...
Protein structure determination is a key step in developing molecular-level understandings of the ro...
Many early protein crystallization trials produce large amounts of small, sometimes sub-diffraction ...
Rational drug design is largely dependent on the successful generation of high-resolution protein st...
The ability to solve a high-resolution protein structure is largely dependent on the successful gene...
Elucidation of the protein structure represents a critical step in the determination of the key func...
A new approach is described to screen for protein nanocrystals based on the reversibility of crystal...
Second order nonlinear optical imaging of chiral crystals (SONICC) has been developed for analyzing ...
Determining protein structure is important for identifying drug targets in order to treat human dise...
The high brilliance of synchrotron facilities have led synchrotron X-ray diffraction to become an in...
Traditional macroscale protein crystallization is accomplished nontrivially by exploring a range of ...
The finding of nonlinear nanometric-sized probes is of key importance for the development of nonline...
The detection and characterization of crystallinity is critical throughout the drug development proc...
Identifying and then optimizing initial crystallization conditions is a prerequisite for m...
International audienceWe have investigated nanocrystals of Fe(IO3)3 by polarization-sensitive second...
There is an increasing demand for rapid, effective methods to identify and detect protein micro- and...
Protein structure determination is a key step in developing molecular-level understandings of the ro...
Many early protein crystallization trials produce large amounts of small, sometimes sub-diffraction ...
Rational drug design is largely dependent on the successful generation of high-resolution protein st...
The ability to solve a high-resolution protein structure is largely dependent on the successful gene...
Elucidation of the protein structure represents a critical step in the determination of the key func...
A new approach is described to screen for protein nanocrystals based on the reversibility of crystal...
Second order nonlinear optical imaging of chiral crystals (SONICC) has been developed for analyzing ...
Determining protein structure is important for identifying drug targets in order to treat human dise...
The high brilliance of synchrotron facilities have led synchrotron X-ray diffraction to become an in...
Traditional macroscale protein crystallization is accomplished nontrivially by exploring a range of ...
The finding of nonlinear nanometric-sized probes is of key importance for the development of nonline...
The detection and characterization of crystallinity is critical throughout the drug development proc...
Identifying and then optimizing initial crystallization conditions is a prerequisite for m...
International audienceWe have investigated nanocrystals of Fe(IO3)3 by polarization-sensitive second...
There is an increasing demand for rapid, effective methods to identify and detect protein micro- and...