Efficency of Improved RAPD Marker In Assesing Genetic Diversity Kayu Kuku (Pericopsis mooniana THW)

Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic diversity on Kayu Kuku (Pericopsis mooniana THW). The findings of this study provides important information for genetic diversity of this highly valuable but rare species of trees. The research was done from August to September 2015 in the Tree Biotechnology and Breeding Laboratory, Faculty of Forestry, Hasanuddin University. DNA from ten genotype P.mooniana was used to amplify with 9 highly polymorphic primers. Genetic diversity analysis was done using Darwin 6.00 software with PCoA and Unweighted Pair Group Method with Aritmatic (UPGMA) method. Results of the investigations indicated the presence of at least 10 alleles in the ten Kayu kuku???s evaluated. Mean number of alleles per locus was 7.1. Results of genetic dissimilarity coefficient calculation and dendogram construction using DARwin 6.00 indicated that the Kayu kuku???s were clustered into three groups. This study genetically characterized the Kayu Kuku species, which might have a significant contribution to the genetic and ecological conservation of this important rare species of trees. Also, this study indicates that the improved RAPD analyses is potential molecular tools for the study of genetic diversity