Antibiotic Sensitivity Pattern of Staphylococcus aureus and Escherichia coli Isolated from bovine Fresh Milk

-The aims of this study were to determine the sensitivity of S. aureus and E. coli isolated from fresh milk against against several antibiotics and to determine the safety of the milk for human comsumsion. Milk was collected from milking diary cow and was used for the bacterial isolation. E. coli were were identified using total plate count (TPC), Gram staining, their growth on Endo Agar and Eosin MethyleneBlue Agar, Bochemical analysis including Glucose,lactose, sucrose, maltose, and sorbitol would be followed by Sorbitol Mac Conkey Agar Test for the identification of E. coli O157:H7.The isolation and identification of S. aureus were performed using Gram stain, TPC, growth on Baird Parker Agar and Mannitol Salt Agar. S. aureus and S. epidermidis were differentiated by coangulase and catalase tests. The antibiotic sensitivity tests for both S. aureus and E. coli were carried out using the following antibiotics: ampicillin, bacitracin, vancomycin, cefoperazone, ceftriaxone, cefotamine, cefuroxime, cefepime, cefazoline, ceftazidime, chloramphenicol, tetracycline, doxycycline, amikacin, kanamycin, neomycin, ertapenem, meropenem, imipenem, erythromycin, gentamycin, nalidixic acid, ciprofloxacin, levofloxacine, norfloxacine, ofloxacin, and novobiocin. Fresh milk obtained from the farm was positive for S. aureus and E. coli and resistant to most antibiotics tested. The best antibiotics for S. aureus were imipenem (54.1mm), ampicillin (42.3 mm) cefazolin (41.6 mm), doxycycline (41.15 mm), and for E.coli were Imipenem (30.1mm), ertapenem (27.9 mm), and meropenem (25.35 mm). The bovine fresh milk examined was contaminated by S. aureus and E. coli and to some extent, were also resistant to most antibiotics tested