The Use of Fungal Rot Isolates and Bio activator in Inhibit Growth of Phytopthora palmivora and Lasiodiplodia theobromae and Decomposition of Cocoa Pod Husks Waste

Cocoa pod husks are major waste of cocoa plants that can be used either as an organic fertilizer or animal feed. For a land area of 972.400 hectares, can produce cocoa beans as much 572.900 tons, and generate waste that reached 1.8766 million tons/year. However, only 94.515 tons of the waste had been used. Very abundantly cocoa biomass in the field if not managed properly will lead to many serious problems such as environmental pollution source (methane, CO2 and NO2) and mainly as the proliferation place of plant pest organisms such as Phytopthora palmivora, Lasiodiplodia theobromae and several kinds of pests. Most farmers are hoarding the crops residues and other wastes around the crop, thus creating optimal conditions for the growth of pathogens.\ud The research was aimed to analyze the use of different fungal rot isolates and bio activator Mikrobat in inhibit the growth of P. palmivora and Lasiodiplodia theobromae in vitro, as well as their role as bio decomposer of cocoa pod husks waste. \ud The result showed, that application of three isolates namely Trichoderma harzianum, Pleurotus ostreatus and Trametes sp. combined with Bio activator derived from bacterial consortium on cocoa pod husks could degrade lignocelluloses levels higher than single application. Comparison of single and combined application of fungal rot isolates and bio activator on cocoa waste was also carried out in the restricted field scale. The results showed that the levels of N, P, and K value in combination treatments tend to be higher than in single application. C/N ratio in the combination treatment was also lower compared with single treatment. \ud The effectiveness of fungal rot isolates and bio activator Mikrobat in inhibiting P. palmivora growth showed that T. harzianum had the best inhibition (59.67%), followed by P. ostreatus (31.72%) and Trametes sp. (28.49%). Similar result was also obtained in the inhibition test against L. theobromae growth. Trichoderma harzianum isolate provide a real inhibition of L. theobromae growth (66.8%), followed by P. ostreatus (40.26%) and Mikrobat (19.74%). \ud Formulations development of bacterial decomposers was made in liquid form, while for fungal decomposer was made in the form of powder using a mixture of bentonite, kaolin and some additional material. The viability test in the first until second months of the storage periods showed no decrease in the growth of the fungal and bacterial active ingredient