Eksplorasi Mikroba Penghasil Enzim Lipolitik pada Sumber Air Panas Lemo Susu, Pinrang, Sulawesi Selatan

Lipase (EC 3.1.1.3) is one of a class of hydrolase enzymes that can hydrolyze triglycerides into fatty acids and glycerol. This study aims to explore the lipolytic enzyme-producing microbes from hot springs Lemo Susu, Pinrang South Sulawesi, by identify microbes generated and determine the optimum time conditions of enzyme production. Production is done by growing microbes on the substrate medium olive oil. Produced lipase activity was tested by titrimetric method. The results obtained showed that the microbial isolates from hot springs Lemo Susu Pinrang, has identified as Bacillus sp. LS3B and Bacillus sp. LS4B. The optimum conditions of production lipolytic Bacillus sp. LS3B and Bacillus sp. LS4B are obtained at fermentation time of 48 hours with successive activity of 0,5310 U/mL and 1,3171 U mL with an optimum temperature of 45 ??C and optimum pH 7