Differential Inhibition of Protein Synthesis in the Nucleus and Cytoplasm of HeLa Cells in the Presence of Actinomycin, Puromycin and Hydroxyurea

A comparison has been made between the synthesis of histones, other nuclear proteins and cytoplasmic proteins after treatment of HeLa cells with substances known to alter their metabolic state: actinomycin, puromycin and hydroxyurea. The use of sensitive methods involving amino acid analysis and measurement of specific radioactivity has revealed differential responses to the action of the different inhibitors. Analysis of these findings as a function of the effects of these inhibitors on other metabolic pathways, suggests that control mechanisms or basic mode of synthesis of some of the histones may differ from that of the other proteins, and that DNA and histone synthesis are probably coordinated in some way yet to be elucidated. Copyright © 1969, Wiley Blackwell. All rights reservedSCOPUS: ar.j