Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: effects of inhibitors of H+ ATPase
- Steinmetz, Philip R.
- Husted, Russell F.
- Beauwens, Renaud
- Mueller, Allan
DOIAbstract
The coupling between H+ transport (JH) and anaerobic glycolysis was examined in vitro in an anaerobic preparation of turtle urinary bladder. JH was measured as the short-circuit current after Na+ transport was abolished with ouabain and by pH stat titration. The media were gassed with N2 and 1% CO2 (PO2<0.5 mm Hg) and contained 10 mm glucose. Under these conditions, JH was not inhibited by 3 mm serosal (S) cyanide or by 0.1 mm mucosal (M) dinitrophenol. Control anerobic lactate production (Jlac) of 47 bladders was plotted as a function of simultaneously measured JH. The slope of Jlac on JH was 0.58±0.12 with an intercept for Jlac at JH=0 of 0.55 μmol/hr. Values for δJlac/δJH were determined in groups of individual bladders when JH was inhib...
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